Saraf^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Saraf^{tm1c(KOMP)Wtsi}
• Name: store-operated calcium entry-associated regulatory factor; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7509470
• Synonyms: SARAF^fl
• Gene: Saraf Location: Chr8:34621733-34638001 bp, + strand Genetic Position: Chr8, 20.9 cM, cytoband A3
• Alliance: Saraf^{tm1c(KOMP)Wtsi} page
• IMPC: Saraf gene page

Mutation origin

• Mutant Cell Line: EPD0613_2_B07
• Germline Transmission: Earliest citation of germline transmission: J:337869
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34631810 of Chromosome 8 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 34631945. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving exon 3 flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:337869)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Saraf Mutation: 22 strains or lines available

References

• Original: J:337869 Gataulin D, et al., Store-operated Ca(2+) entry regulatory factor alters murine metabolic state in an age-dependent manner via hypothalamic pathways. PNAS Nexus. 2023 Mar;2(3):pgad068
• All: 1 reference(s)