Zfp276^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Zfp276^{tm1c(EUCOMM)Hmgu}
• Name: zinc finger protein (C2H2 type) 276; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7510125
• Synonyms: Zfp276^fl
• Gene: Zfp276 Location: Chr8:123980934-123996484 bp, + strand Genetic Position: Chr8, 72.09 cM
• Alliance: Zfp276^{tm1c(EUCOMM)Hmgu} page
• IMPC: Zfp276 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:324574
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 123982195 of Chromosome 8 upstream of the critical exons 2 to 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 123985267. Exons 2 to 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:324574)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp276 Mutation: 28 strains or lines available

References

• Original: J:324574 Aberle T, et al., Transcription factor Zfp276 drives oligodendroglial differentiation and myelination by switching off the progenitor cell program. Nucleic Acids Res. 2022 Feb 28;50(4):1951-1968
• All: 1 reference(s)