Kpna2^em1Alfon
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kpna2^em1Alfon
• Name: karyopherin subunit alpha 2; endonuclease-mediated mutation 1, Alfonso Gutierez-Adan
• MGI ID: MGI:7512791
• Synonyms: Kpna2^-4
• Gene: Kpna2 Location: Chr11:106879455-106890351 bp, - strand Genetic Position: Chr11, 70.08 cM
• Alliance: Kpna2^em1Alfon page

Mutation origin

• Strain of Origin: (C57BL/6 x CBA)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 4-bp (TGTT) deletion in exon 2 predicted to result in a frameshift and early chain termination due to a premature stop codon and generate a 30 amino acid protein instead of the 529 amino acid wild-type protein. Western blot and immunofluorescence analysis confirmed absence of protein in testes. This allele represents line 4. (J:338393)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kpna2 Mutation: 43 strains or lines available

References

• Original: J:338393 Navarrete-Lopez P, et al., Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors. iScience. 2023 Jul 21;26(7):107134
• All: 1 reference(s)