Nmu^{tm1.1(cre)Debu}
Targeted Allele Detail

Summary

• Symbol: Nmu^{tm1.1(cre)Debu}
• Name: neuromedin U; targeted mutation 1.1, Dimitri De Bundel
• MGI ID: MGI:7512918
• Synonyms: Nmu-Cre, NmuCre-IRES-Nmu
• Gene: Nmu Location: Chr5:76481342-76511624 bp, - strand Genetic Position: Chr5, 40.93 cM
• Alliance: Nmu^{tm1.1(cre)Debu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338487
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (No functional change, Recombinase)
• Mutation: Insertion
• Nmu^{tm1.1(cre)Debu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nmu (MGI:1860476)

• Mutation details: A targeting construct was designed to insert a Cre coding sequence, an IRES element, the coding sequence of NMU isoform 1, an hGHpolyA signal, and a neomycin cassette for positive selection, flanked by two flippase recognition target (FRT) sites in frame with exon 1 ATG of the Nmu gene on chromosome 5 maintaining the expression of the most prevalent isoform of the Nmu gene products. After homologous recombination in ES cells, resulting chimeras were crossed to Flp deleter mice to excise the neo-cassette. (J:338487)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nmu (mouse) Summary of all recombinase alleles driven by Nmu. Alliance mouse Nmu gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nmu Mutation: 20 strains or lines available

References

• Original: J:338487 Medrano M, et al., Neuroanatomical characterization of the Nmu-Cre knock-in mice reveals an interconnected network of unique neuropeptidergic cells. Open Biol. 2023 Jun;13(6):220353
• All: 1 reference(s)