Syt13^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Syt13^{tm1d(EUCOMM)Hmgu}
• Name: synaptotagmin XIII; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7514188
• Synonyms: Syt13^FD
• Gene: Syt13 Location: Chr2:92745446-92786403 bp, + strand Genetic Position: Chr2, 51.37 cM, cytoband E1
• Alliance: Syt13^{tm1d(EUCOMM)Hmgu} page
• IMPC: Syt13 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327292
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 92770636 of Chromosome 2 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 92771553. The critical exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:327292)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syt13 Mutation: 22 strains or lines available

References

• Original: J:327292 Bakhti M, et al., Synaptotagmin-13 orchestrates pancreatic endocrine cell egression and islet morphogenesis. Nat Commun. 2022 Aug 4;13(1):4540
• All: 1 reference(s)