Gnat2^{tm1.1(cre/ERT2)Eye}
Targeted Allele Detail

Summary

• Symbol: Gnat2^{tm1.1(cre/ERT2)Eye}
• Name: G protein subunit alpha transducin 2; targeted mutation 1.1, Stephen H Tsang
• MGI ID: MGI:7514880
• Synonyms: Gnat2^CreERT2
• Gene: Gnat2 Location: Chr3:108000105-108008748 bp, + strand Genetic Position: Chr3, 46.83 cM
• Alliance: Gnat2^{tm1.1(cre/ERT2)Eye} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338407
• Parent Cell Line: KV1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Gnat2^{tm1.1(cre/ERT2)Eye} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gnat2 (MGI:95779)

• Mutation details: A CreERT2 with an FRT-flanked neomycin cassette replaced the translation START codon (ATG) in exon 2 of the Gnat2 gene by homologous recombination in the ES cells. Male chimeras were bred to ACTB-FLPe females for germline transmission and neomycin cassette removal. (J:338407)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gnat2 (mouse) Summary of all recombinase alleles driven by Gnat2. Comparative matrix view of recombinase activities Alliance mouse Gnat2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnat2 Mutation: 35 strains or lines available

References

• Original: J:338407 Wang NK, et al., Spatiotemporal control of genome engineering in cone photoreceptors. Cell Biosci. 2023 Jun 28;13(1):119
• All: 1 reference(s)