Gt(ROSA)26Sor^{tm14.1(Thy1-dre)Maoh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm14.1(Thy1-dre)Maoh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 14.1, Masato Ohtsuka
• MGI ID: MGI:7516494
• Synonyms: Thy1-Dre
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm14.1(Thy1-dre)Maoh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303408
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm14.1(Thy1-dre)Maoh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Thy1 (MGI:98747)

• Mutation details: Recombinase-mediated cassette exchange (RMCE) replaced the expression cassette between the JT15 and lox2272 sites of Gt(ROSA)26Sor^tm1Maoh with Thy1-Dre cassette. Flp-mediated recombination removed the extra sequence downstream of the Thy1-Dre in the construct flanked by FRT sites. Lines 1-3 are established exhibiting similar recombination activity patterns. This allele is an representative of all three lines. (J:303408)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Thy1 (mouse) Summary of all recombinase alleles driven by Thy1. Comparative matrix view of recombinase activities Alliance mouse Thy1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:303408 Tamura S, et al., Thy1 promoter activity in the Rosa26 locus in mice: lessons from Dre-rox conditional expression system. Exp Anim. 2020 Aug 5;69(3):287-294
• All: 1 reference(s)