Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj
Transgene Detail

Summary

• Symbol: Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj
• Name: transgene insertion, John Yu
• MGI ID: MGI:7516772
• Synonyms: Puf-A shRNA
• Transgene: Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj Location: unknown
• Alliance: Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj page

Transgene origin

• Strain of Origin: Not Applicable

Transgene description

• Transgene Type: Transgenic (Inducible, Knockdown)
• Mutation: Insertion
• Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj involves 1 genes/genome features (Pum3) View all
• Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: A construct containing a Pgk promoter driving a tetracycline repressor gene followed by a poly a sequence and a tetracycline responsive Pol III hybrid promoter driving expression of Pum3 shRNAs. Treatment with doxycycline results in about an 80% reduction in Pum3 mRNA expression in hemizygous embryos. (J:338852)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:338852 Ko CF, et al., The Puf-A Protein Is Required for Primordial Germ Cell Development. Cells. 2022 Apr 27;11(9)
• All: 1 reference(s)