Samd11^em1Tfur
Endonuclease-mediated Allele Detail

Summary

• Symbol: Samd11^em1Tfur
• Name: sterile alpha motif domain containing 11; endonuclease-mediated mutation 1, Takahisa Furukawa
• MGI ID: MGI:7520290
• Gene: Samd11 Location: Chr4:156331423-156340717 bp, - strand Genetic Position: Chr4, Syntenic
• Alliance: Samd11^em1Tfur page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303740
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 5 to 11 were replaced with a loxP site and FRT-flanked PGK-neomycin cassette via CRISPR/Cas9 technology. RT-PCR, Western blot, and immunostaining confirmed the absence of mRNA and protein in retinal sections. (J:303740)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Samd11 Mutation: 22 strains or lines available

References

• Original: J:303740 Kubo S, et al., Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity. Sci Rep. 2021 Feb 18;11(1):4180
• All: 1 reference(s)