Gt(ROSA)26Sor^{em1.1(CAG-EGFP*)Maoh}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1.1(CAG-EGFP*)Maoh}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, Masato Ohtsuka
• MGI ID: MGI:7525863
• Synonyms: Gt(ROSA)26Sor^{em1(CAG-EGFP[*1])Maoh}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1.1(CAG-EGFP*)Maoh} page

Mutation origin

• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Insertion
• Mutation details: The targeting construct inserted an FRT site, CAG promoter driving ubiquitous expression of an EGFP reporter gene, FRT site, and lox2272 site. Further CRISPR/Cas9 genome editing introduced a 1bp deletion in the EGFP gene, eliminating expression of the reporter. (J:340084)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

References

• Original: J:340084 Miura H, et al., Novel reporter mouse models useful for evaluating in vivo gene editing and for optimization of methods of delivering genome editing tools. Mol Ther Nucleic Acids. 2021 Jun 4;24:325-336
• All: 2 reference(s)