Alpi^{tm1.1(cre/ERT2)Cle}
Targeted Allele Detail

Summary

• Symbol: Alpi^{tm1.1(cre/ERT2)Cle}
• Name: alkaline phosphatase, intestinal; targeted mutation 1.1, Hans Clevers
• MGI ID: MGI:7531286
• Synonyms: Alpi^CreER
• Gene: Alpi Location: Chr1:87025724-87029328 bp, - strand Genetic Position: Chr1, 44.05 cM, cytoband C5
• Alliance: Alpi^{tm1.1(cre/ERT2)Cle} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:340289
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Alpi^{tm1.1(cre/ERT2)Cle} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Alpi (MGI:1924018)

• Mutation details: An IRES-cre/ERT2 cassette followed by a Frt-flanked neo-selection cassette was inserted into exon 11 of the Alpi gene by homologous recombination in ES cells. Correctly targeted ES cell clones were injected into C57BL/6 blastocysts. Mice were crossed to Flp-expressing mice to delete the neo cassette. (J:340289)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Alpi (mouse) Summary of all recombinase alleles driven by Alpi. Comparative matrix view of recombinase activities Alliance mouse Alpi gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Alpi Mutation: 28 strains or lines available

References

• Original: J:340289 Tetteh PW, et al., Replacement of Lost Lgr5-Positive Stem Cells through Plasticity of Their Enterocyte-Lineage Daughters. Cell Stem Cell. 2016 Feb 4;18(2):203-13
• All: 2 reference(s)