Nsf^tm1Himat
Targeted Allele Detail

Summary

• Symbol: Nsf^tm1Himat
• Name: N-ethylmaleimide sensitive fusion protein; targeted mutation 1, Hideo Matsuzaki
• MGI ID: MGI:7531287
• Gene: Nsf Location: Chr11:103712608-103844882 bp, - strand Genetic Position: Chr11, 67.54 cM
• Alliance: Nsf^tm1Himat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:313900
• Parent Cell Line: EGR-101 (ES Cell)
• Strain of Origin: C57BL/6NSlc

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector PG00174_Z_5_D06 obtained from KOMP inserted between exons 5 and 6 and is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 6-7 at position 103914303. The critical exons 6-7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:313900)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nsf Mutation: 153 strains or lines available

References

• Original: J:313900 Xie MJ, et al., Autistic-Like Behavior and Impairment of Serotonin Transporter and AMPA Receptor Trafficking in N-Ethylmaleimide Sensitive Factor Gene-Deficient Mice. Front Genet. 2021;12:748627
• All: 1 reference(s)