Tg(Flt1-cre/ERT2)1Tmak
Transgene Detail

Summary

• Symbol: Tg(Flt1-cre/ERT2)1Tmak
• Name: transgene insertion 1, Taija Makinen
• MGI ID: MGI:7532647
• Synonyms: Flt1-CreERT2, Vegfr1-CreERT2
• Transgene: Tg(Flt1-cre/ERT2)1Tmak Location: unknown
• Alliance: Tg(Flt1-cre/ERT2)1Tmak page

Transgene origin

• Strain of Origin: C57BL/6NTac

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Flt1-cre/ERT2)1Tmak expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Flt1 (MGI:95558)

• Mutation details: A BAC (B6Ng01-247E8) carrying the mouse Flt1 gene was used to generate a transgene by replacing the coding sequence in exon 1 as well as the splice donor site at the junction between exon 1 and intron 1 (50 bp) with a cassette containing the open reading frame for CreERT2 and the Flt1 3' untranslated region. A polyadenylation signal (human growth hormone polyadenylation signal) was inserted 3' of the Flt1 3'UTR sequence. Two independent founder lines were generated, of which one (line 1) showed efficient Cre-mediated recombination and was used for further analyses. (J:341130)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Flt1 (mouse) Summary of all recombinase alleles driven by Flt1. Comparative matrix view of recombinase activities Alliance mouse Flt1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:341130 Petkova M, et al., Immune-interacting lymphatic endothelial subtype at capillary terminals drives lymphatic malformation. J Exp Med. 2023 Apr 3;220(4)
• All: 1 reference(s)