Prtn3^{tm1.1(cre/ERT2,CD4*)Wkas}
Targeted Allele Detail

Summary

• Symbol: Prtn3^{tm1.1(cre/ERT2,CD4*)Wkas}
• Name: proteinase 3; targeted mutation 1.1, Wolfgang Kastenmuller
• MGI ID: MGI:7539266
• Synonyms: Prtn3^CreERT2-hCD4
• Gene: Prtn3 Location: Chr10:79715499-79719008 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Prtn3^{tm1.1(cre/ERT2,CD4*)Wkas} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341387
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Prtn3^{tm1.1(cre/ERT2,CD4*)Wkas} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prtn3 (MGI:893580)

• Mutation details: An IRES-CreERT2-IRES-humanCD4-FRT-neo-FRT construct was inserted into the 5th exon of the endogenous Prtn3 gene after the endogenous stop codon. The Neomycin cassette used for selection was then removed by the flippase enzyme. The human CD4 coding sequence used in the construct lacks the C-terminal 33 amino acids and contains the F43I mutation, generating a mutated and truncated protein that precludes signaling and association with MHCII. (J:341387)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prtn3 (mouse) Summary of all recombinase alleles driven by Prtn3. Comparative matrix view of recombinase activities Alliance mouse Prtn3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prtn3 Mutation: 13 strains or lines available

References

• Original: J:341387 Ugur M, et al., Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networks. Immunity. 2023 Aug 8;56(8):1778-1793.e10
• All: 1 reference(s)