Mlc1^{tm2.1(cre/ERT2)Jhmc}
Targeted Allele Detail

Summary

• Symbol: Mlc1^{tm2.1(cre/ERT2)Jhmc}
• Name: megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human); targeted mutation 2.1, Joseph H McCarty
• MGI ID: MGI:7540321
• Synonyms: MLCT
• Gene: Mlc1 Location: Chr15:88840087-88863192 bp, - strand Genetic Position: Chr15, 44.46 cM
• Alliance: Mlc1^{tm2.1(cre/ERT2)Jhmc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341452
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129 x C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Mlc1^{tm2.1(cre/ERT2)Jhmc} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mlc1 (MGI:2157910)

• Mutation details: The T2A-CreERT2 cassette was inserted immediately upstream of the endogenous stop codon in the exon 12 of the Mlc1 gene in frame. The FRT flanked Neo selection cassette was inserted 116 bp downstream of the 3' UTR. The Neo cassette in the targeting vector was removed during ES clone expansion. (J:341452)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mlc1 (mouse) Summary of all recombinase alleles driven by Mlc1. Comparative matrix view of recombinase activities Alliance mouse Mlc1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mlc1 Mutation: 28 strains or lines available

Notes

The targeting vector was transfected by electroporation of FLP 129/SvEv x C57Bl/6 (HF4) embryonic stem (ES) cells.

References

• Original: J:341452 Morales JE, et al., Mlc1-Expressing Perivascular Astrocytes Promote Blood-Brain Barrier Integrity. J Neurosci. 2022 Feb 23;42(8):1406-1416
• All: 1 reference(s)