Glipr1l1^em1Mkob
Endonuclease-mediated Allele Detail

Summary

• Symbol: Glipr1l1^em1Mkob
• Name: GLI pathogenesis-related 1 like 1; endonuclease-mediated mutation 1, Moira K O'Bryan
• MGI ID: MGI:7541080
• Gene: Glipr1l1 Location: Chr10:111896094-111914415 bp, + strand Genetic Position: Chr10, 60.07 cM, cytoband D1
• Alliance: Glipr1l1^em1Mkob page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 7-bp deletion (ACTATCA) in exon 1, which is predicted to result in a premature stop codon in exon 1 and a frame-shift mutation. qPCR analysis shows a 92% reduction in mRNA levels in isolated testis and germ cells from homozygotes. Immunofluorescent labeling confirmed absence of protein in sperm. (J:290694)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Glipr1l1 Mutation: 23 strains or lines available

References

• Original: J:290694 Gaikwad AS, et al., GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse. BMC Biol. 2019 Oct 31;17(1):86
• All: 1 reference(s)