Htr2a^{em1(cre/ERT2)Blr}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Htr2a^{em1(cre/ERT2)Blr}
• Name: 5-hydroxytryptamine (serotonin) receptor 2A; endonuclease-mediated mutation 1, Bryan L Roth
• MGI ID: MGI:7543065
• Synonyms: Htr2a^EGFP-CreERT2, Htr2a-EGFP-CT-IRES-CreERT2, Htr2a^{em1(EGFP, creERT2)Blr}
• Gene: Htr2a Location: Chr14:74878314-74944299 bp, + strand Genetic Position: Chr14, 39.37 cM
• Alliance: Htr2a^{em1(cre/ERT2)Blr} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Htr2a^{em1(cre/ERT2)Blr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Htr2a (MGI:109521)

• Mutation details: A cDNA encoding EGFP (flanked on both ends by a serine- and glycine- rich linker) was inserted into the C-terminus (CT) of the mouse Htr2a gene after residue 452 (encoded by exon 3; chr14:74706337 in the GRCm38/mm10 genome assembly). An IRES-CreERT2 cassette was placed between the stop codon and the 3' UTR (chr14:74706397). The mutation was created using CRISPR/cas9 methodologies in C57BL/6J embryos. (J:341620)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Htr2a (mouse) Summary of all recombinase alleles driven by Htr2a. Alliance mouse Htr2a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Htr2a Mutation: 43 strains or lines available

References

• Original: J:341620 Chiu YT, et al., A suite of engineered mice for interrogating psychedelic drug actions. bioRxiv. 2023 Sep 26
• All: 1 reference(s)