Hand2^em1(dre)Pjen
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hand2^em1(dre)Pjen
• Name: heart and neural crest derivatives expressed 2; endonuclease-mediated mutation 1, Patricia Jensen
• MGI ID: MGI:7543292
• Synonyms: Hand2Dre
• Gene: Hand2 Location: Chr8:57774018-57777552 bp, + strand Genetic Position: Chr8, 29.8 cM
• Alliance: Hand2^em1(dre)Pjen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:347747
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Hand2^em1(dre)Pjen expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hand2 (MGI:103580)

• Mutation details: A 2A peptide, Dre recombinase cDNA and SV40 poly(A) cassette were targeted to exon 2 of Hand2 by Crispr/Cas9 genome editing. Homologous recombination resulted in replacement of the Hand2 stop codon and 73 bp of 3' UTR with the 2A/Dre/polyA cassette. (J:347747)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hand2 (mouse) Summary of all recombinase alleles driven by Hand2. Comparative matrix view of recombinase activities Alliance mouse Hand2 gene page

Expression

In Mice Carrying this Mutation:

32 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hand2 Mutation: 12 strains or lines available

References

• Original: J:347747 Plummer NW, et al., A knock-in allele of Hand2 expressing Dre recombinase. Genesis. 2024;62(3):e23601
• All: 1 reference(s)