Gt(ROSA)26Sor^{tm1(Mib1*V943F)Jlp}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Mib1*V943F)Jlp}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Jose de la Pompa
• MGI ID: MGI:7543648
• Synonyms: R26MIB1^V943F
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Mib1*V943F)Jlp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338889
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The following elements were inserted in an intron of the gene: a loxP site, a neomycin resistance gene and STOP cassette, a second loxP site, sequence coding for an HA tag in-frame with the coding sequence for Mib1 with a p.V943F mutation, IRES sequence and the EGFP fluorescent reporter gene. The inserted Mib1 and EGFP genes will only be expressed after Cre-mediated removal of the neo-STOP cassette. (J:338889)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:338889 Siguero-Alvarez M, et al., A Human Hereditary Cardiomyopathy Shares a Genetic Substrate With Bicuspid Aortic Valve. Circulation. 2023 Jan 3;147(1):47-65
• All: 1 reference(s)