Tnk2^tm1.2Npma
Targeted Allele Detail

Summary

• Symbol: Tnk2^tm1.2Npma
• Name: tyrosine kinase, non-receptor, 2; targeted mutation 1.2, Nupam P Mahajan
• MGI ID: MGI:7543727
• Synonyms: Ack1^KO
• Gene: Tnk2 Location: Chr16:32462699-32502311 bp, + strand Genetic Position: Chr16, 23.16 cM
• Alliance: Tnk2^tm1.2Npma page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341740
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked PGK-neomycin selection cassette and loxP site were inserted upstream of exon 3 and a second loxP site was inserted downstream of exon 3. The PGK-neomycin selection cassette was removed via flp-mediated recombination. Exon 3 was removed via cre-mediated recombination and generated a stop codon TGG due to splicing of exon 2 with exon 4, causing premature termination. (J:341740)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnk2 Mutation: 62 strains or lines available

References

• Original: J:341740 Sridaran D, et al., Inhibiting ACK1-mediated phosphorylation of C-terminal Src kinase counteracts prostate cancer immune checkpoint blockade resistance. Nat Commun. 2022 Nov 14;13(1):6929
• All: 1 reference(s)