Bdnf^tm1.1Tmiz
Targeted Allele Detail

Summary

• Symbol: Bdnf^tm1.1Tmiz
• Name: brain derived neurotrophic factor; targeted mutation 1.1, Toshiyuki Mizui
• MGI ID: MGI:7545595
• Synonyms: BDNF^pro
• Gene: Bdnf Location: Chr2:109505045-109557352 bp, + strand Genetic Position: Chr2, 56.63 cM
• Alliance: Bdnf^tm1.1Tmiz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303078
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Insertion
• Mutation details: The coding region in the last exon was replaced with the same fragment coding for a cleavage-resistant form with p.R135M (c.404G>T) and p.R137L (c.410G>T) mutations, and a loxP site flanked neomycin resistance gene cassette was inserted downstream of the gene. The neo cassette was removed through subsequent Cre-mediated recombination. The mutations, close to the encoded peptide cleavage site, affect proteolytic cleavage of the prodomain that is necessary to form the mature peptide. They mimic the human tandem SNPs c.374G>T (rs1048220) and c.380G>T (rs1048221). (J:303078)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bdnf Mutation: 42 strains or lines available

References

• Original: J:303078 Kojima M, et al., Reduction in BDNF from Inefficient Precursor Conversion Influences Nest Building and Promotes Depressive-Like Behavior in Mice. Int J Mol Sci. 2020 Jun 1;21(11):3984
• All: 1 reference(s)