Pdcl2^em1Ncvc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pdcl2^em1Ncvc
• Name: phosducin-like 2; endonuclease-mediated mutation 1, National Cerebral and Cardiovascular Center
• MGI ID: MGI:7546801
• Gene: Pdcl2 Location: Chr5:76459963-76479003 bp, - strand Genetic Position: Chr5, 40.88 cM
• Alliance: Pdcl2^em1Ncvc page

Mutation origin

• Strain of Origin: C57BL/6 x DBA/2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology using gRNAs 5-AAAAGATGAAATTGAAGAGA-3 for the second exon and 5-ATTTATGACAGCGATAGCTC-3 for the sixth exon, generated a 12,565-bp deletion of exons 2-6. Western blot analysis confirmed absence of protein in testicular germ cells of homozygotes. (J:338084)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdcl2 Mutation: 21 strains or lines available

References

• Original: J:338084 Fujihara Y, et al., PDCL2 is essential for sperm acrosome formation and male fertility in mice. Andrology. 2023 Jul;11(5):789-798
• All: 1 reference(s)