Birc3^em1Haka
Endonuclease-mediated Allele Detail

Summary

• Symbol: Birc3^em1Haka
• Name: baculoviral IAP repeat-containing 3; endonuclease-mediated mutation 1, Hamid Kashkar
• MGI ID: MGI:7560638
• Synonyms: cIAP2 mutant RING, cIap2^MutR
• Gene: Birc3 Location: Chr9:7848700-7873187 bp, - strand Genetic Position: Chr9, 2.46 cM, cytoband A2
• Alliance: Birc3^em1Haka page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated an 8-bp deletion leading to a frameshift that prolongs the mutant protein by 23 amino acids changing the phenylalanine at position 600 to a leucine (G601QfsTer34). This deletion was generated by simultaneously injecting C57BL/6N zygotes with two sgRNAs targeting Birc2 (5-TCTGCAGGGGGACAATCAAG-3) and Birc3 (5-ATCTGTAGAGGGACCATCAA-3) and was generated at the same time as the Birc2^em1Haka allele. (J:342656)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Birc3 Mutation: 32 strains or lines available

Notes

This allele was generated at the same time as Birc2^em1Haka.

References

• Original: J:342656 Schorn F, et al., cIAPs control RIPK1 kinase activity-dependent and -independent cell death and tissue inflammation. EMBO J. 2023 Nov 15;42(22):e113614
• All: 1 reference(s)