Swhtr^tm1.1Phg
Targeted Allele Detail

Summary

• Symbol: Swhtr^tm1.1Phg
• Name: sweetheart long non-coding RNA; targeted mutation 1.1, Phillip Grote
• MGI ID: MGI:7565401
• Synonyms: Swhtr^3xpA
• Gene: Swhtr Location: unknown Genetic Position: Chr17, Syntenic
• Alliance: Swhtr^tm1.1Phg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342171
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A FRT-flanked PGK-Neo-SV40pA selection cassette inserted a strong transcriptional stop signal (3xpA) downstream of a phylogenetically conserved region of the GATA4 bound first heart field specific enhancer. The selection cassette was removed via flp-mediated recombination. This insertion causes a premature termination of transcription and a severely shortened transcript. RNA-seq and qPCR confirmed the absence of full-length RNA in E9.5 hearts. The expression level of its cis located gene Nkx2-5 is unchanged in E9.5 hearts. And the lncRNAs IRENE-SS and IRENE-div expression levels are not affected. (J:342171)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Swhtr Mutation: 0 strains or lines available

Notes

ES cells for generating the allele are either G4 (129S6/SvEvTac x C57BL/6NTac)F1 or C57BL/6J (J:342171).

References

• Original: J:342171 Rogala S, et al., The lncRNA Sweetheart regulates compensatory cardiac hypertrophy after myocardial injury in murine males. Nat Commun. 2023 Nov 2;14(1):7024
• All: 1 reference(s)