Per1^tm1Ual
Targeted Allele Detail

Summary

• Symbol: Per1^tm1Ual
• Name: period circadian clock 1; targeted mutation 1, University at Albany
• MGI ID: MGI:7568833
• Synonyms: Per1^loxP
• Gene: Per1 Location: Chr11:68986043-69000786 bp, + strand Genetic Position: Chr11, 42.37 cM, cytoband B
• Alliance: Per1^tm1Ual page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342930
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A cassette containing neomycin acetyl-transferase (neo) flanked by two FRT sites was inserted into a bacterial artificial chromosome encompassing the Per1 locus (clone bMQ-339m04). Two loxP sites flanking exon 4-16 of Per1 were introduced to delete exons 4-16 and the reading frame could be resumed in exon 22, thereby deleting almost the entire gene. The neo cassette for selection in ES cells was between exon 3 in the short arm of homology (1.4 kb) and the loxP site upstream of exon 4. The loxP site downstream of exon 16 was followed by the long arm of homology (4 kb) containing exons 17-22. (J:342930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Per1 Mutation: 63 strains or lines available

References

• Original: J:342930 Olejniczak I, et al., Light affects behavioral despair involving the clock gene Period 1. PLoS Genet. 2021 Jul;17(7):e1009625
• All: 1 reference(s)