Chrna6^{tm2(flpo)Tasic}
Targeted Allele Detail

Summary

• Symbol: Chrna6^{tm2(flpo)Tasic}
• Name: cholinergic receptor, nicotinic, alpha polypeptide 6; targeted mutation 2, Bosiljka Tasic
• MGI ID: MGI:7571383
• Synonyms: Chrna6-IRES2-FlpO-neo
• Gene: Chrna6 Location: Chr8:27893240-27903972 bp, - strand Genetic Position: Chr8, 16.02 cM, cytoband A3
• Alliance: Chrna6^{tm2(flpo)Tasic} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Chrna6^{tm2(flpo)Tasic} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Chrna6 (MGI:106213)

• Mutation details: The targeting vector contains, from 5' to 3', a partial Chrna6 sequence within exon 6 up to and including the endogenous stop codon, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a FlpO recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an AttP site. (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Chrna6 (mouse) Summary of all recombinase alleles driven by Chrna6. Alliance mouse Chrna6 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrna6 Mutation: 30 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)