Grb7^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Grb7^{tm1d(EUCOMM)Wtsi}
• Name: growth factor receptor bound protein 7; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7572892
• Synonyms: Grb7^-, Grb7^del
• Gene: Grb7 Location: Chr11:98337660-98346199 bp, + strand Genetic Position: Chr11, 61.75 cM
• Alliance: Grb7^{tm1d(EUCOMM)Wtsi} page
• IMPC: Grb7 gene page

Mutation origin

• Mutant Cell Line: EPD0543_2_C04
• Germline Transmission: Earliest citation of germline transmission: J:344139
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98341112 of Chromosome 11 upstream of the critical exons 2-7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 7 at position 98343225. Exons 2-7 are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exons. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:344139)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grb7 Mutation: 19 strains or lines available

References

• Original: J:344139 Lofgren KA, et al., Grb7 knockout mice develop normally but litters born to knockout females fail to thrive. Dev Dyn. 2023 Dec 23
• All: 1 reference(s)