Casp8^em1Hbz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Casp8^em1Hbz
• Name: caspase 8; endonuclease-mediated mutation 1, Haibing Zhang
• MGI ID: MGI:7572923
• Synonyms: Casp8^deltaE385
• Gene: Casp8 Location: Chr1:58834553-58886663 bp, + strand Genetic Position: Chr1, 29.19 cM, cytoband B
• Alliance: Casp8^em1Hbz page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Intragenic deletion
• Mutation details: Three nucleotides (AAG) were deleted from exon 8, effectively deleting glutamic acid codon 385 (p.E385delE, GRCm39:chr1:g.58883848_58883850delAAG), using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The deletion is in the auto-cleavage site of the encoded peptide and affects its enzymatic activity by blocking auto-cleavage. (J:342732)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casp8 Mutation: 45 strains or lines available

References

• Original: J:342732 Li X, et al., Caspase-8 auto-cleavage regulates programmed cell death and collaborates with RIPK3/MLKL to prevent lymphopenia. Cell Death Differ. 2022 Aug;29(8):1500-1512
• All: 1 reference(s)