Tnrc18^em1Ggw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tnrc18^em1Ggw
• Name: trinucleotide repeat containing 18; endonuclease-mediated mutation 1, Gang Greg Wang
• MGI ID: MGI:7578774
• Synonyms: Tnrc18^{W2745A/Y2747A}
• Gene: Tnrc18 Location: Chr5:142710416-142803417 bp, - strand Genetic Position: Chr5, 81.73 cM
• Alliance: Tnrc18^em1Ggw page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Tryptophan codon 2563 (TGG) and tyrosine codon 2565 (TAC) in exon 23 were changed to alanine (GCC, GCC) (ENSMUSP00000117651:p.W2563A, p.Y2565A) using an sgRNA (targeting GTGGTTCTACCATCCGG) and an ssODN template with CRISPR/Cas9 technology. The mutations are the equivalent of the human p.W2858A and p.Y2860A mutations and render the encoded peptide H3K9me3-binding-defective. (J:342966)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnrc18 Mutation: 85 strains or lines available

References

• Original: J:342966 Zhao S, et al., TNRC18 engages H3K9me3 to mediate silencing of endogenous retrotransposons. Nature. 2023 Nov;623(7987):633-642
• All: 1 reference(s)