Mtif3^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mtif3^{tm1d(EUCOMM)Wtsi}
• Name: mitochondrial translational initiation factor 3; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7580965
• Gene: Mtif3 Location: Chr5:146888383-146900610 bp, - strand Genetic Position: Chr5, 86.57 cM
• Alliance: Mtif3^{tm1d(EUCOMM)Wtsi} page
• IMPC: Mtif3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287609
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt0 cassette was inserted at position 146895365 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 146896572. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287609)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mtif3 Mutation: 20 strains or lines available

References

• Original: J:287609 Rudler DL, et al., Fidelity of translation initiation is required for coordinated respiratory complex assembly. Sci Adv. 2019 Dec;5(12):eaay2118
• All: 2 reference(s)