Tubb4b^{tm2b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tubb4b^{tm2b(EUCOMM)Wtsi}
• Name: tubulin, beta 4B class IVB; targeted mutation 2b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7593955
• Gene: Tubb4b Location: Chr2:25112172-25114714 bp, - strand Genetic Position: Chr2, 17.08 cM, cytoband A3
• Alliance: Tubb4b^{tm2b(EUCOMM)Wtsi} page
• IMPC: Tubb4b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:344732
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 25113751 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 25114464. The critical exon(s) is/are thus flanked by loxP sites. Cre mediated recombination without flp expression, created a null reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:344732)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tubb4b Mutation: 18 strains or lines available

References

• Original: J:344732 Sewell MT, et al., Tubb4b is required for multi-ciliogenesis in the mouse. Development. 2023 Nov 30;:dev.201819
• All: 1 reference(s)