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Cyb5r1tm1c(KOMP)Wtsi
Targeted Allele Detail
Summary
Symbol: Cyb5r1tm1c(KOMP)Wtsi
Name: cytochrome b5 reductase 1; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID: MGI:7596535
Synonyms: Cyb5r1fl
Gene: Cyb5r1  Location: Chr1:134333519-134339476 bp, + strand  Genetic Position: Chr1, 58.21 cM
Alliance: Cyb5r1tm1c(KOMP)Wtsi page
IMPC: Cyb5r1 gene page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:344225
Parent Cell Line:  JM8A1.N3 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready)
Mutation:    Insertion
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 134335033 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 134336511. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:344225)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cyb5r1 Mutation:  32 strains or lines available
References
Original:  J:344225 Faber JE, et al., Large differences in collateral blood vessel abundance among individuals arise from multiple genetic variants. J Cereb Blood Flow Metab. 2023 Nov;43(11):1983-2004
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory