Orm2^em1Bcgen
Endonuclease-mediated Allele Detail

Summary

• Symbol: Orm2^em1Bcgen
• Name: orosomucoid 2; endonuclease-mediated mutation 1, Biocytogen LLC
• MGI ID: MGI:7607189
• Gene: Orm2 Location: Chr4:63280686-63284115 bp, + strand Genetic Position: Chr4, 33.96 cM
• Alliance: Orm2^em1Bcgen page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9-mediated recombination using 2 guide RNAs targeting upstream of exon 1 and downstream of exon 6 created a deletion spanning exons 1-6. Western blot analysis confirmed the absence of protein expression in livers from homozygous male mice. (J:333118)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Orm2 Mutation: 12 strains or lines available

References

• Original: J:333118 Zhou B, et al., Orosomucoid 2 maintains hepatic lipid homeostasis through suppression of de novo lipogenesis. Nat Metab. 2022 Sep;4(9):1185-1201
• All: 1 reference(s)