Gpcpd1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gpcpd1^{tm1c(EUCOMM)Hmgu}
• Name: glycerophosphocholine phosphodiesterase 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7610680
• Gene: Gpcpd1 Location: Chr2:132371002-132420173 bp, - strand Genetic Position: Chr2, 64.57 cM, cytoband F3
• Alliance: Gpcpd1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Gpcpd1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:345630
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 132388494 of Chromosome 2 upstream of the critical exons, 9 and 10 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 10 at position 132390080. The critical exons are thus flanked by loxP sites. A "conditional ready" (floxed) allele was be created by flp recombinase recombination of this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:345630)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpcpd1 Mutation: 32 strains or lines available

References

• Original: J:345630 Cikes D, et al., Gpcpd1-GPC metabolic pathway is dysfunctional in aging and its deficiency severely perturbs glucose metabolism. Nat Aging. 2024 Jan;4(1):80-94
• All: 2 reference(s)