Emb^tm1Heij
Targeted Allele Detail

Summary

• Symbol: Emb^tm1Heij
• Name: embigin; targeted mutation 1, Jyrki Heino
• MGI ID: MGI:7611846
• Gene: Emb Location: Chr13:117357109-117410951 bp, + strand Genetic Position: Chr13, 65.4 cM
• Alliance: Emb^tm1Heij page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:345100
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 117400482 of Chromosome 13 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 117401282. Exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Western blot and PCR analysis confirmed the absence of expression in kidneys from homozygous mice. (J:345100)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Emb Mutation: 38 strains or lines available

References

• Original: J:345100 Talvi S, et al., Embigin deficiency leads to delayed embryonic lung development and high neonatal mortality in mice. iScience. 2024 Feb 16;27(2):108914
• All: 1 reference(s)