Arfip2^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Arfip2^{tm1d(EUCOMM)Wtsi}
• Name: ARF interacting protein 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7611851
• Gene: Arfip2 Location: Chr7:105283410-105289623 bp, - strand Genetic Position: Chr7, 55.92 cM, cytoband F1
• Alliance: Arfip2^{tm1d(EUCOMM)Wtsi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:345639
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 105286613 of Chromosome 7 upstream of the critical exons 4-5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 4-5 at position 105287999. The critical exons 4-5 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene creating a conditional ready (floxed) allele. Exons 4-5 were removed via cre-mediated recombination in the germline generating a knock-out mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:345639)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arfip2 Mutation: 21 strains or lines available

References

• Original: J:345639 Guo H, et al., ADP-Ribosylation Factor-Interacting Protein 2 Acts as a Novel Regulator of Mitophagy and Autophagy in Podocytes in Diabetic Nephropathy. Antioxidants (Basel). 2024 Jan 8;13(1)
• All: 1 reference(s)