Tmem263^em1Gww
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmem263^em1Gww
• Name: transmembrane protein 263; endonuclease-mediated mutation 1, Guang William Wong
• MGI ID: MGI:7612228
• Gene: Tmem263 Location: Chr10:84938491-84953611 bp, + strand Genetic Position: Chr10, 41.63 cM
• Alliance: Tmem263^em1Gww page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology using gRNAs 5-CTATCATATGTCTCTCATTAG-3 AND 5-GCCACTGGCATCATATGCTG-3 deleted exon 3 that encodes approximately 81% of the full-length protein. qPCR confirmed the absence of transcript in the liver and hypothalamus of homozygotes. (J:345905)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem263 Mutation: 9 strains or lines available

References

• Original: J:345905 Sarver DC, et al., Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition. Elife. 2024 Jan 19;12
• All: 1 reference(s)