Mustn1^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mustn1^{tm1d(EUCOMM)Wtsi}
• Name: musculoskeletal, embryonic nuclear protein 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7616867
• Gene: Mustn1 Location: Chr14:30601214-30603567 bp, + strand Genetic Position: Chr14, 19.09 cM
• Alliance: Mustn1^{tm1d(EUCOMM)Wtsi} page
• IMPC: Mustn1 gene page

Mutation origin

• Mutant Cell Line: EPD0917_3_F04
• Germline Transmission: Earliest citation of germline transmission: J:346460
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 30602117 of Chromosome 14 upstream of the critical exon 2 and part of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 30603225. The critical exon 2 and part of exon 3 are thus flanked by loxP sites. The lacZ sequence and neo selection cassette were removed via flp-mediated recombination. Exon 2 and part of exon 3 were removed via cre-mediated recombination in the germline. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:346460)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mustn1 Mutation: 9 strains or lines available

References

• Original: J:346460 Ducommun S, et al., Mustn1 is a smooth muscle cell-secreted microprotein that modulates skeletal muscle extracellular matrix composition. Mol Metab. 2024 Mar 6;82:101912
• All: 1 reference(s)