Gnb1^em2Dgold
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gnb1^em2Dgold
• Name: guanine nucleotide binding protein (G protein), beta 1; endonuclease-mediated mutation 2, David B Goldstein
• MGI ID: MGI:7620476
• Synonyms: Gnb1^Del71
• Gene: Gnb1 Location: Chr4:155575818-155643726 bp, + strand Genetic Position: Chr4, 86.17 cM
• Alliance: Gnb1^em2Dgold page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 6 was targeted for a lysine codon 78 to arginine (AGA) (NM_008142:c.233A>G:p.K78R) mutation using an sgRNA and an ssODN template with CRISPR/Cas9 technology. This allele has an un-targeted 71 bp deletion including part of exon 6. (J:346983)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnb1 Mutation: 61 strains or lines available

References

• Original: J:346983 Colombo S, et al., Epilepsy in a mouse model of GNB1 encephalopathy arises from altered potassium (GIRK) channel signaling and is alleviated by a GIRK inhibitor. Front Cell Neurosci. 2023;17:1175895
• All: 1 reference(s)