Mtarc1^tm1.1Vlcg
Targeted Allele Detail

Summary

• Symbol: Mtarc1^tm1.1Vlcg
• Name: mitochondrial amidoxime reducing component 1; targeted mutation 1.1, Velocigene
• MGI ID: MGI:7623614
• Synonyms: Marc1^-
• Gene: Mtarc1 Location: Chr1:184518964-184543394 bp, - strand Genetic Position: Chr1, 89.07 cM, cytoband H4
• Alliance: Mtarc1^tm1.1Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:346201
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 were replaced with a lacZ reporter, loxP site, self-deleting protamine promoter, cre recombinase, neomycin selection cassette and second loxP site using Regenerons VelociGene technology. The neomycin selection marker located between two loxP sites was deleted with cre recombinase under self-deleting protamine promoter, leaving beta-galactosidase gene (LacZ) and one loxP site in the flanking intron. Western blot analysis confirmed the absence of protein in liver lysates. (J:346201)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mtarc1 Mutation: 16 strains or lines available

References

• Original: J:346201 Smagris E, et al., Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse. PLoS Genet. 2024 Mar;20(3):e1011179
• All: 1 reference(s)