Xdh^tm2.1Takni
Targeted Allele Detail

Summary

• Symbol: Xdh^tm2.1Takni
• Name: xanthine dehydrogenase; targeted mutation 2.1, Takeshi Nishino
• MGI ID: MGI:7624143
• Synonyms: XDH ki, Xdh^tm2Nms
• Gene: Xdh Location: Chr17:74190890-74257191 bp, - strand Genetic Position: Chr17, 45.25 cM
• Alliance: Xdh^tm2.1Takni page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:281671
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Cysteine codon 995 (TGT) in exon 27 was changed to arginine (CGG) (p.C995R) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 28. The neo cassette was removed through subsequent Cre-mediated recombination. The mutation stabilizes the encoded peptide in the xanthine dehydrogenase (XDH) form so that there is a reduced likelihood that it will be converted into the xanthine oxidase (XO) form. XO activity represents <20% of total activity in liver homogenates. (J:281671)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Xdh Mutation: 101 strains or lines available

References

• Original: J:281671 Kusano T, et al., Targeted knock-in mice expressing the oxidase-fixed form of xanthine oxidoreductase favor tumor growth. Nat Commun. 2019 Oct 28;10(1):4904
• All: 3 reference(s)