Orai3^em1.1Treb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Orai3^em1.1Treb
• Name: ORAI calcium release-activated calcium modulator 3; endonuclease-mediated mutation 1.1, Mohamed Trebak
• MGI ID: MGI:7625689
• Synonyms: Orai3^fl
• Gene: Orai3 Location: Chr7:127368987-127374322 bp, + strand Genetic Position: Chr7, 69.73 cM
• Alliance: Orai3^em1.1Treb page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 mediated recombination using guide RNAs inserted an FRT flanked trapping cassette (splice acceptor-Bgeo-pA) in the intron upstream of exon 3 followed by a floxed exon 3. Flp-mediated recombination removed the trapping cassette. (J:345485)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Orai3 Mutation: 18 strains or lines available

References

• Original: J:345485 Emrich SM, et al., Orai3 and Orai1 mediate CRAC channel function and metabolic reprogramming in B cells. Elife. 2023 Feb 21;12
• All: 1 reference(s)