Igsf3^em1Laap
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igsf3^em1Laap
• Name: immunoglobulin superfamily, member 3; endonuclease-mediated mutation 1, Pirjo Laakkonen
• MGI ID: MGI:7626255
• Gene: Igsf3 Location: Chr3:101284399-101370375 bp, + strand Genetic Position: Chr3, 44.3 cM
• Alliance: Igsf3^em1Laap page

Mutation origin

• Strain of Origin: FVB/NRj

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 146 bp deletion in exon 3, resulting in a premature stop codon right after the signal peptide. qPCR confirmed the absence of mRNA in homozygous P2.5 cerebrum and intestinal tissue. Western blot analysis shows nearly complete loss of protein expression in homozygotes. (J:341537)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igsf3 Mutation: 56 strains or lines available

References

• Original: J:341537 Tanjore Ramanathan J, et al., Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization. Sci Rep. 2023 Oct 11;13(1):17162
• All: 1 reference(s)