Mpc1^tm1.2Mwol
Targeted Allele Detail

Summary

• Symbol: Mpc1^tm1.2Mwol
• Name: mitochondrial pyruvate carrier 1; targeted mutation 1.2, Michael J Wolfgang
• MGI ID: MGI:7628164
• Synonyms: Mpc1^D
• Gene: Mpc1 Location: Chr17:8502590-8516499 bp, + strand Genetic Position: Chr17, 4.92 cM
• Alliance: Mpc1^tm1.2Mwol page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236179
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP sites were inserted around exons 3-5 and an IRES-nls-eYFP, lox511 flanked stop signal, luciferase gene and FRT flanked selection cassette were inserted downstream of exon 5 via homologous recombination. Flp mediated recombination removed the selection cassette. Cre mediated recombination reomved exons 3-5 and the lox511 flanked stop signal creating a null allele and allowing expression of luciferase. (J:236179)

Expression

In Mice Carrying this Mutation:

42 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mpc1 Mutation: 16 strains or lines available

References

• Original: J:236179 Bowman CE, et al., Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series. Mol Cell Biol. 2016 Aug 1;36(15):2089-104
• All: 1 reference(s)