Nfatc1^tm1.1Fbs
Targeted Allele Detail

Summary

• Symbol: Nfatc1^tm1.1Fbs
• Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1; targeted mutation 1.1, Friederike Berberich-Siebelt
• MGI ID: MGI:7639766
• Synonyms: Nfatc1^deltaSUMO
• Gene: Nfatc1 Location: Chr18:80649420-80756286 bp, - strand Genetic Position: Chr18, 53.66 cM, cytoband E4
• Alliance: Nfatc1^tm1.1Fbs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305649
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (BALB/c x C57BL/6)F1

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Lysine codons 689 (AAG) and 896 (AAG) in exon 9 were changed to arginine (CGG) (ENSMUSP00000077196:p.K689R, ENSMUSP00000126884:p.K896R). A loxP site was inserted into intron 8 and a loxP site flanked neomycin resistance gene cassette was inserted into intron 9. The neo cassette was removed through subsequent Cre-mediated recombination. The mutations prevent sumoylation of the two affected residues in the encoded peptide. Cre-mediated deletion of the floxed exon 9 would specifically prevent the translation of a full-length betaC isoform. (J:305649)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfatc1 Mutation: 49 strains or lines available

References

• Original: J:305649 Xiao Y, et al., Lack of NFATc1 SUMOylation prevents autoimmunity and alloreactivity. J Exp Med. 2021 Jan 4;218(1)
• All: 1 reference(s)