Slc28a1^em1Govr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Slc28a1^em1Govr
• Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 1; endonuclease-mediated mutation 1, Rajgopal Govindarajan
• MGI ID: MGI:7643463
• Gene: Slc28a1 Location: Chr7:80764547-80820164 bp, + strand Genetic Position: Chr7, 45.71 cM
• Alliance: Slc28a1^em1Govr page

Mutation origin

• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 mediated mutation using guide RNAs targeting exon 2 created a 5 bp deletion (ATGGC) in exon 2 resulting in a frameshift mutation and an AG to CT mutation of an AcuI restriction site. Densitometric quantification showed an over 95% reduction in immunoreactivity in tissues from homozygous mice. (J:336148)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc28a1 Mutation: 41 strains or lines available

References

• Original: J:336148 Persaud AK, et al., Increased renal elimination of endogenous and synthetic pyrimidine nucleosides in concentrative nucleoside transporter 1 deficient mice. Nat Commun. 2023 Jun 1;14(1):3175
• All: 1 reference(s)