Marveld3^em2Cepi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Marveld3^em2Cepi
• Name: MARVEL (membrane-associating) domain containing 3; endonuclease-mediated mutation 2, Christophe E Pierreux
• MGI ID: MGI:7645616
• Synonyms: MD3^-
• Gene: Marveld3 Location: Chr8:110674546-110688835 bp, - strand Genetic Position: Chr8, 57.38 cM
• Alliance: Marveld3^em2Cepi page

Mutation origin

• Strain of Origin: C57BL/6 x DBA/2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology using crRNA 5-TGTCGCCCTCGGATCGTTTC-3 and crRNA 5-GGACCAGGGGACCGCCGAGGG-3 deleted approximately 686 bp, encompassing the transcription initiation site, the first exon and part of the first intron. While RT-PCR showed 90% reduction in the expression of both isoform-coding mRNAs in E15.5 pancreas, Western blot analysis showed absence of protein in adult pancreatic and renal extracts. (J:307816)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Marveld3 Mutation: 23 strains or lines available

References

• Original: J:307816 Heymans C, et al., Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function. Sci Rep. 2021 Jul 15;11(1):14519
• All: 1 reference(s)