Dnmt3l^em1Hsas
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnmt3l^em1Hsas
• Name: DNA methyltransferase 3-like; endonuclease-mediated mutation 1, Hiroyuki Sasaki
• MGI ID: MGI:7646871
• Synonyms: Dnmt3L^ADD
• Gene: Dnmt3l Location: Chr10:77878121-77899456 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Dnmt3l^em1Hsas page

Mutation origin

• Strain of Origin: (C57BL/6N x C3H/HeN)F1 x C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Aspartic acid codon 124 (GAT) in exon 6 was changed to alanine (GCT) (p.D124A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation, in the ADD domain of the encoded peptide, reduces the affinity of the peptide to bind to H3K4me0. (J:347433)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3l Mutation: 49 strains or lines available

References

• Original: J:347433 Kubo N, et al., Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells. Nat Commun. 2024 Apr 16;15(1):3266
• All: 1 reference(s)