Dnmt3a^em1Hsas
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnmt3a^em1Hsas
• Name: DNA methyltransferase 3A; endonuclease-mediated mutation 1, Hiroyuki Sasaki
• MGI ID: MGI:7646874
• Synonyms: Dnmt3a^ADA, Dnmt3a^ADD
• Gene: Dnmt3a Location: Chr12:3856007-3964443 bp, + strand Genetic Position: Chr12, 1.99 cM, cytoband A2-A3
• Alliance: Dnmt3a^em1Hsas page

Mutation origin

• Strain of Origin: (C57BL/6N x C3H/HeN)F1 x C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Aspartic acid codons 525 (GAC) and 527 (GAT) in exon 14 were changed to alanine (GCC, GCT) (p.D525A, p.D527A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutations, in the ADD domain of the encoded peptide, reduce the affinity of the peptide to bind to H3K4me0. (J:349016)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3a Mutation: 139 strains or lines available

References

• Original: J:349016 Uehara R, et al., The DNMT3A ADD domain is required for efficient de novo DNA methylation and maternal imprinting in mouse oocytes. PLoS Genet. 2023 Aug;19(8):e1010855
• All: 2 reference(s)