Eif4h^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Eif4h^{tm1c(EUCOMM)Wtsi}
• Name: eukaryotic translation initiation factor 4H; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7646937
• Gene: Eif4h Location: Chr5:134648726-134668263 bp, - strand Genetic Position: Chr5, 74.71 cM
• Alliance: Eif4h^{tm1c(EUCOMM)Wtsi} page
• IMPC: Eif4h gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:344730
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 134653071 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 134657095. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:173534, J:344730)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eif4h Mutation: 34 strains or lines available

References

• Original: J:344730 Screen M, et al., RNA helicase EIF4A1-mediated translation is essential for the GC response. Life Sci Alliance. 2024 Feb;7(2)
• All: 2 reference(s)